IMMUNOHISTOCHEMISTRY, HMB 45

What this test checks

Detects HMB45, a melanoma-associated antigen found in melanocytes and melanoma cells. Used to confirm melanoma diagnosis and identify melanoma cells in metastatic deposits.

Why do people get this test?

HMB45 is one of the classic melanoma markers. It helps confirm melanoma when histological appearances are unclear and identifies melanoma cells in lymph nodes or distant metastases — critical for staging and treatment.

When you should consider this test

• When melanoma diagnosis needs confirmation
• When metastatic melanoma is suspected in a tissue sample
• As part of melanoma diagnostic panel

How to prepare

No preparation needed — performed on biopsy or surgical tissue.