HISTOPATHOLOGY DIRECT IMMUNOFLUORESCENCE (DIF) SKIN / CONJUNCTIVAL BIOPSY PANEL

What this test checks

Examines skin or conjunctival tissue under fluorescence microscopy to detect antibody deposits — the hallmark of autoimmune blistering diseases like pemphigus, bullous pemphigoid, and mucous membrane pemphigoid.

Why do people get this test?

Direct immunofluorescence is the gold standard for diagnosing autoimmune blistering diseases. The pattern of antibody deposits under fluorescence gives a definitive diagnosis that guides immunosuppressive treatment.

When you should consider this test

• When you have blistering of the skin, mouth, or eyes of unclear cause
• When autoimmune skin disease is suspected
• When diagnosis of pemphigus, pemphigoid, or dermatitis herpetiformis is being considered

How to prepare

No preparation needed — the small skin sample is taken by your dermatologist.